Troponin T Cardiac antibody

Cat.# 4T19 / 4T19cc

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  • Cat.#

    4T19 / 4T19cc

  • MAbs in vitro (Cat.#4T19cc)

     300cc, 329cc, 406cc, 1C11cc, 1F11cc

     

  • MAbs in vivo (Cat.#4T19)

     9G6, 7F4, 7G7, 2F3, 1A11, 7E7

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic human TnT peptide a.a.r. 119-138 conjugated with carrier protein (300cc and 329cc), or synthetic human TnT peptide a.a.r. 106-183 conjugated with carrier protein (406cc), free human cTnT (2F3, 7G7, 1A11, 9G6, 1C11cc, 1F11cc, 7F4) or human Tn complex (7E7).

  • Specificity:

     406cc, 1C11cc, 1F11cc, 2F3, 7F4, 7G7, 1A11 and 9G6 have no cross-reaction with skeletal TnT.Cross-reactivity of 300cc and 329cc with skeletal TnT is less than 1%. Cross-reactivity of 7E7 with cardiac TnI is 5 %.

     

  • MAb isotypes:

    IgG1 for 300cc, 329cc, 1C11cc, 9G6, 7G7, 7E7

    IgG2a for 406cc

    IgG2b for 1F11cc 2F3, 1A11, 7F4

     

  • Applications:

     

    TnT immunoassay. Recommended pairs for sandwich immunoassay (capture – detection):

    406cc – 300cc

    329cc – 406cc

    1C11cc and 1F11cc are recommended for Western blotting.

     

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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Troponin T Cardiac antibody ()
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