Recombinant human cardiac troponin T (cTnT)

Cat.# 8RTT5

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  • Cat.#

    8RTT5

  • Synonym names

    Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3

  • Source:

    Expressed in E. coli

  • Protein sequence:

    Product of gene TNNT2; 288 a.a.r.

    Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).
    For full sequence, see printable -file.

  • Application:

    Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard

  • Analysis:

    Purity > 95 % (SDS-PAGE)

    Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.490 (measured at 280 nm)

  • Immunologicalﾠ activity:ﾠ

    Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform

  • Purification:

    Immunoaffinity purification followed by ion-exchange chromatography

  • Presentation:

    Lyophilized from 0.01 M HCl

    It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.

  • Storage:

    Lyophilized -20°C (-15 … -30 °C allowed)
    Reconstituted -70 °C (-65 … -80 °C allowed)

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

  • Other information:

    Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

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Recombinant human cardiac troponin T (cTnT)

(Catalogue number and MAb: 8RTT5)

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Recombinant human cardiac troponin T (cTnT)

(Catalogue number and MAb: 8RTT5)

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