Recombinant feline serum amyloid A (SAA)

8FS5

Expressed in E. coli.

Recombinant SAA consists of 121 amino acids and has a predicted molecular mass of 13838 kDa.

Consists of a polypeptide corresponding to 1-111 a.a.r. of feline SAA and a proprietary 10 a.a.r. affinity tag on the N-terminus.

Can be used as a control in immunoassays.

Purity >95 % (Tricine-SDS-PAGE).

Concentration is determined spectrophotometrically

Affinity and reversed-phase chromatography

Lyophilized from 0.01 M HCl (pH 2.0)

It is recommended to reconstitute this product with 0.01 M HCl (pH 2.0) to 0.1-1 mg/ml concentration.

Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)

Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

To prepare diluted samples of SAA it is recommended to use buffer solutions containing a detergent. The selection of an appropriate detergent and its concentration is essential for the assay performance.

For sandwich immunoassays using monoclonal anti-SAA antibodies SAA19, SAA21 and VSA34 we recommend using 0.01% CHAPS in antigen dilution buffer (e.g. 0.05 M Tris-HCl, pH 8.0, 0.15 M NaCl, 0.01 % CHAPS).

For sandwich immunoassays using monoclonal anti-SAA antibodies VSA25 and VSA31 we recommend using 0.05 % Tween 20 in antigen dilution buffer (e.g. 0.02 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.05 % Tween 20).

This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.