PAPP-A, human, recombinant

Cat.# 8PA1

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  • unit

    mg

  • Cat.#

    8PA1

  • Source: 

    Expressed in a mammalian cell line.

  • Description:

    Synthetic DNA fragment encoding amino acids 82 – 1627 of human pregnancy-associated protein A (PAPP-A, Accession number UniProtKB #Q13219; PAPP1_HUMAN) with E483A mutation was used for recombinant protein expression; homodimer.

    Contains C-terminal His10-tag.

    Theoretical MW: 173762.91 Da including C-terminal His10-tag

    Theoretical pI: 5.56

  • Application: 

    Immunoassays

  • Analysis: 

    Purity >90%

    Concentration is determined spectrophotometrically at A280. Abs 1 mg/ml = 1.34

  • Purification: 

    Metal affinity chromatography

  • Presentation: 

    Lyophilized from 20 mM Tris-HCl, 0.15 M NaCl, 5% sucrose, pH 7.5

    It is recommended to reconstitute this product with deionized water to its initial concentration.

  • Storage: 

    Lyophilized at -20 °C (-15 … -30 °C allowed), reconstituted at -70 °C (-65 … -80 °C allowed)

  • Other information: 

    Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

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PAPP-A, human, recombinant

(Catalogue number and MAb: 8PA1)

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PAPP-A, human, recombinant

(Catalogue number and MAb: 8PA1)

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