4N6
5G10, 5E2, 1C1, H11
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.
Human NSE
IgG2a for MAbs 5E2, 1C1, H11
IgG2b for MAb 5G10
MAbs are working in ELISA, Western blotting and in immunohistochemistry on formalin-fixed and paraffin-embedded sections.
Recommended pairs for sandwich immunoassay (capture-detection):
5G10 – 1C1
5E2 – 1C1
1C1 – 5E2
Protein G chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4N6-)