Insulin-like growth factor binding protein 4 (IGFBP-4) antibody

Cat.# 4IGF4

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  • Cat.#

    4IGF4

  • MAbs in vitro:

    IBP3cc

  • MAbs in vivo:

    IBP144, IBP154, IBP163, IBP180, IBP182, IBP185, IBP190,

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human IGFBP-4 expressed in mammalian cell line (MAbs IBP144, IBP154, IBP180, IBP182, IBP185, IBP190), synthetic peptide from human IGFBP-4 His142-Met156 (MAbs IBP3cc) or synthetic peptide from human IGFBP-4 Lys157-Gln171 (MAb IBP163)

  • Specificity:

    MAbs IBP144, IBP154 and IBP180: human IGFBP-4; human N-terminal IGFBP-4 fragment (NT-IGFBP-4)
    MAbs IBP182, IBP185 and IBP190: human IGFBP-4; human C-terminal IGFBP-4 fragment (CT-IGFBP-4)
    MAbs IBP3cc: human NT-IGFBP-4, cross-reaction with full-length human IGFBP-4 <5% (ELISA)
    MAb IBP163: human CT-IGFBP-4, cross-reaction with full-length human IGFBP-4 <5% (ELISA)

  • MAb isotypes:

    IgG1 for MAbs IBP163, IBP190
    IgG2a for MAbs IBP144, IBP154, IBP180
    IgG2b for MAbs IBP182, IBP185
    IgG3 for MAbs IBP3, IBP3cc

  • Applications:

    Detection of human IGFBP-4, NT-IGFBP-4, and CT-IGFBP-4. MAbs are working in direct ELISA and sandwich immunoassay.

    Recommended pairs for human IGFBP-4 sandwich immunoassay (capture-detection):
    IBP185 – IBP154
    IBP182 – IBP144
    IBP144 – IBP190

    Recommended pairs for specific human NT-IGFBP-4 sandwich immunoassay (capture-detection):
    IBP3cc – IBP144
    IBP3cc – IBP180

    Recommended pairs for specific human CT-IGFBP-4 sandwich immunoassay (capture-detection):
    IBP182 – IBP163

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for MAbs IBP144, IBP154, IBP163, IBP180, IBP182, IBP185, IBP190

    50 mM citrate, 150 mM NaCl, pH 6.0, 0,09% azide (NaN₃) for MAb IBP3cc

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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Insulin-like growth factor binding protein 4 (IGFBP-4) antibody

(Catalogue number and MAb: 4IGF4-)

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Insulin-like growth factor binding protein 4 (IGFBP-4) antibody

(Catalogue number and MAb: 4IGF4-)

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