Influenza A nucleoprotein, antibody

3IN5

FA32, FA35, FA38, FA58

Recombinant antibody expressed in a mammalian cell line. Full-size IgG sequence derived from rabbit B cells.

FA52

Recombinant chimeric antibody expressed in a mammalian cell line. Composed of original wild type variable domains of rat derived MAb and human IgG1 constant domains.

FA91, FA94

Recombinant chimeric antibody expressed in a mammalian cell line. Composed of original wild type variable domains of sheep derived MAb and human IgG1 constant domains.

FA17

Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

F8, InA108, InA180, InA224, InA245

Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

Recombinant nucleoprotein of influenza virus type A strain H1N1 A/California/07/2009 for FA17, FA32, FA35, FA38, FA52, FA58, FA91, FA94

Purified influenza virus type A strain H1N1 for F8, InA108, InA180, InA224, InA245

Influenza virus type A (nucleoprotein). No cross-reactivity to NP of influenza B virus.
The following strains were tested with MAbs FA17, FA32, FA35, FA38, FA52, FA58, FA91, and FA94 and they all reacted with these strains:

Testing was carried out using corresponding recombinant nucleoproteins or lysates of purified viral preparations.
All antibodies are not cross-reactive to influenza B virus (<0.1%). Testing was carried out using lysates of purified viral preparations of influenza B/Colorado/06/2017.
MAbs FA17, FA32, FA35, FA38, FA52, FA58, FA91, FA94 were tested with SARS-CoV-2 nucleoprotein and demonstrated no cross-reaction (<0.05%)

IgG for FA32, FA35, FA38, FA58

IgG1 for FA17, FA52, FA91, FA94, InA108, InA224

IgG2a for F8

IgG2b for InA245

IgG3 for InA180

MAb F8 can be used in immunocytochemistry. MAb F8 inhibits viral reproduction after fatty acid acylation.

MAbs InA108 and InA245 detect influenza A nucleoprotein in Western blotting.

All MAbs are working in ELISA.

Recommended pairs for influenza A nucleoprotein detection: 

Protein A chromatography for FA17, FA32, FA35, FA38, FA52, FA58, FA91, FA94, InA108, InA180, InA224, InA245

Protein G chromatography for F8

PBS, pH 7.4, 0.09 % sodium azide (NaN3) for FA17, FA32, FA35, FA38, FA52, FA58, FA91, FA94, F8, InA108, InA224, InA245

50 mM sodium citrate, 150 mM NaCl, pH 6.0, 0,09 % azide (NaN3) for InA180

+4 °C (+2 … +8 °C allowed)

This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.