Monoclonal mouse anti-human osteocalcin

Cat.# 4OC8

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  • Cat.#

    4OC8

  • MAbs in vitro:

    2H9cc, 6F9cc, 1C4, 1C7, 3G8, 8H12

    Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

  • MAbs in vivo:

    3G7

    Mouse monoclonal antibody. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

  • Immunogen:

    Bovine osteocalcin with keyhole limpet hemocyanin for 3G7, 1C4, 1C7, 3G8
    Recombinant human osteocalcin with glutathione S-transferase for 2H9cc, 6F9cc, 8H12

  • Specificity:

    2H9cc cross-reacts with bovine, rat and pig OC and is not cross-reacting with mouse OC.

    6F9cc is not cross-reacting with bovine, rat, mouse, rabbit or pig OC.

    The ability of the MAbs to recognize full length human osteocalcin, tryptic 1-19, 20-43, synthetic 7-19, 15-31 and bovine osteocalcin was tested with Eu-labelled antigens. *3G8 recognizes unlabelled human osteocalcin (hOC) and bovine osteocalcin (bOC) when tested with two-site combinations.

  • MAb isotypes:

    IgG1 for 1C4, 1C7, 3G8, 6F9cc, 8H12
    IgG2a for 2H9cc
    IgG2b for 3G7

  • Applications:

    MAbs are working in ELISA. Recommended pairs are:

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN3 ) for 6F9cc, 2H9cc

    50 mM sodium citrate, 150 mM NaCl, pH 6.0, 0.09 % sodium azide (NaN3 ) for 1C4, 1C7, 3G8, 8H12

    0.9 % NaCl, 0.09 % sodium azide (NaN3 ) for 3G7

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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Monoclonal mouse anti-human osteocalcin

(Catalogue number and MAb: 4OC8-)

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Monoclonal mouse anti-human osteocalcin

(Catalogue number and MAb: 4OC8-)

Evaluation samples available. You can save up to 50%*

Information

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