HCG (Human chorionic gonadotropin) Antibody

Cat.# 2H8

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  • Cat.#

    2H8

  • MAbs in vitro:

    F1cc

    Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

  • MAbs in vivo

    28A4, 27E8, 77F12

    Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

  • Immunogen:

    Purified hCG alpha chain for F1cc

    Native human HCG (whole molecule) for 28A4, 27E8, 77F12

  • Specificity:

    MAbs 28A4 and 27E8 specifically recognize beta-subunit of HCG. MAbs are not cross-reacting with hLH, hTSH, hFSH.

    MAb 77F12 specifically recognizes HCG; it reacts mainly with alpha-subunit. MAb is cross-reacting with whole molecule of HCG, hLH, hTSH and hFSH. Cross-reactivity with free beta-subunit of HCG was not detected.

    MAb F1cc is specific for the alpha-chain of FSH, LH, TSH and HCG.

  • MAb isotypes:

    IgG1 for MAbs 27E8, F1cc
    IgG2a for MAb 28A4
    IgG2b for MAbs 77F12

  • Applications:

    Detection of HCG in immunoassays. Suitable for immunochromatography (not tested with F1cc).

    Best recommended pairs for sandwich immunoassay (capture-detection):
    77F12 - 28A4, 27E8 - 28A4

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN3)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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HCG (Human chorionic gonadotropin) Antibody ()
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