Hormone Antibodies

Cortisol antibody

Cat.# 2C2 / 2C2cc

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Cat.#

2C2 / 2C2cc
MAbs in vitro (Cat.# 2C2cc)

XM210cc
Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of
Sp2/0 myeloma cells with spleen cells of Balb/c mice.
MAbs in vivo (Cat.# 2C2)

CORT-2
Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of
Sp2/0 myeloma cells with spleen cells of Balb/c mice.
MAbs in vivo (Cat.# 2C2)

CORT-2

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with conjugate of purified cortisol with BSA.
Immunogen:

Purified cortisol conjugated with BSA

Specificity:

React with cortisol-BSA conjugate and free cortisol, no cross-reactivity with BSA.
Cross-reactivity, % 50% displacement of cortisol-3-125-Iodine:

Steroid	XM210cc	CORT-2
Cortisol	100	100
Dexamethasone	20	N/A
11-Deoxycortisol	0.9	N/A
Prednisolone	5.6	N/A
Corticosterone	0.6	49
11-Deoxycorticosterone	<0.1	N/A
Progesterone	<0.1	N/A
17-Hydroxyprogesterone	<0.1	0
Testosterone, Estradiol, Estriol	<0.1	N/A
Danazol	<0.01	N/A

MAb isotypes:

IgG2 for MAb XM210cc

IgG3 for MAb CORT-2
Applications:

Detection of cortisol in competitive assay.
Purification:

Protein A chromatography
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for XM210cc
50 mM sodium citrate, 150 mM NaCl, pH 6.0, 0.09 % sodium azide (NaN₃) for CORT-2
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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Cortisol antibody

Cat.# 2C2 / 2C2cc

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