CA-125 antibody

Cat.# 4C29

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  • Cat.#

    4C29

  • Recombinant MAbs:

    RX16
    Recombinant antibody expressed in a mammalian cell line. Composed of original wild type variable domains of mouse derived MAb and mouse IgG1 constant domains.

  • MAbs in vitro:

    X306cc, X52cc
    Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

  • MAbs in vivo:

    X75, X325
    Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

  • Immunogen:

    CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa)

  • Specificity:

    CA125 antigen.

    MAbs X52cc; X75, X325: epitope specificity group B (ISOBM classification) similar to M-11.

    MAb X306cc, RX16: epitope specificity group A (ISOBM classification) similar to OC125.

  • MAb isotypes:

    IgG1 for RX16, X306cc, X52cc, X75, X325

  • Applications:

    Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection):

    RX16 – X52cc
    RX16 – X325
    RX16 – X75
    X306cc – X52cc
    X306cc – X325
    X306cc – X75

    All MAbs are working in Western blotting. MAb X325 can be used in immunohistochemistry on paraffin embedded tissue.

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN3 ) for RX16, X306cc, X52cc
    50 mM sodium citrate, 150 mM NaCl, pH 6.0, 0.09 % sodium azide (NaN3) for X75, X325

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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