mg
8H11
Expressed in E.coli
Recombinant antigen
HIV-1 V3 domains of gp120 from groups M and O, ectodomain of gp41 from group M and ectodomain fragment of gp41 from group O
Contains N-terminal Trx-tag and a C-terminal His10-tag
Theoretical molecular weight of the whole construct 56.98 kDa
Theoretical pI 5.93.
Purity ≥80 %
Concentration is determined spectrophotometrically
Metal affinity chromatography
50 mM NaH₂PO₄, 0.24 M NaCl, pH 7.4, 10 mM KCl, 0.5 M Arg, 0.4 M sucrose, 1 mM EDTA,
3 M Urea, 5 mM 2-mercaptoethanol
-70 °C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
Product should not be stored unfrozen.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8H11)